Abdulkadir KESKİN

Article | 2017 | South African Journal of Animal Science47 ( 4 )

Heat stress proteins are important factors in protecting cells against environmental stress. The HSP70.1 gene is one of the most important members of the heat stress protein family, which is essential for life, production and reproduction. In this study, partial regions of HSP70.1 (3' and 5' untranslated regions (UTRs)) were sequenced in six cattle breeds. Blood samples of five native breeds, namely Yerli Kara (YK), Boz irk (BI), Yerli Guney Sarisi (YGS), Guney Dogu Anadolu Kirmizisi (GAK) and Dogu Anadolu Kirmizisi (DAK) were collected from their native regions and blood samples of the Holstein Friesian (Siyah Alaca (SA)) breed wer . . .e collected from each of these regions. Totals of 249 and 206 animals were analysed for the HSP70.1-3' and 5' UTR regions, respectively. In the 3' UTR region, 13 single nucleotide polymorphisms (SNPs) and one indel were found, whereas this region was found to be monomorphic among animals of the Holstein Friesian breed. In the 5' UTR region, 43 SNPs and three indels were revealed in all of the investigated breeds. On the other hand, a new C983T nucleotide substitution was identified in this region, and is thought to disrupt the Sp1-hsp70 promoter binding site. The 5' UTR region was also more variable in the Turkish native breeds than in the Holstein Friesian. This study is the first to investigate the 3' and 5' UTRs of the HSP70.1 gene in Turkish native breeds. The genetic structure of these gene regions in Turkish native cattle breeds was found to be quite different from those of other cattle breeds that had been studied in the past More less

Fatih Ramazan İSTANBULLUGİL | Ali RIŞVANLI | Ruslan SALIKOV | Ulaş ACARÖZ | Damla ARSLAN ACARÖZ

Article | 2023 | South African Journal of Animal Science53 ( 6 )

The aim of the present study was to determine the relationship of immunoglobulins and cytokine levels in the milk of Kyrgyz mares during the last period of lactation. For this purpose, seven Kyrgyz mares were used. During the last week of lactation, milk samples were obtained from mares four times at intervals of two days. Physicochemical tests were performed on all collected milk samples, and pH, fat, ash, titratable acidity, and dry matter were measured. Interferon-gamma (IFN-γ), interleukin-2 (IL-2), tumour necrosis factor-alpha (TNF-α), and immunoglobulin G (IgG) levels were determined using commercial enzyme-linked immunosorben . . .t assay (ELISA) kits. Based on the measurements, the lowest IgG level was determined on the last day of lactation (10.23 ± 13.13 µg/mL) and the TNF-α level was determined to be the highest on the last day lactation (61.10 ± 75.69 pg/mL). However, no statistical differences between the days in terms of IFN-γ, IL-2, pH, titratable acidity, dry matter, ash, and fat were found. Varying degrees of positive correlations between the parameters existed and were more pronounced between IgG levels and pH values. As a result, it was concluded that in the last period of lactation in Kyrgyz mares, IgG concentrations decreased and TNF-α levels increased in their milk, but substantial changes in milk composition, immunoglobulins, and cytokine levels in milk did not show positive correlations with the physicochemical properties of milk. Keyword: cytokine; immunoglobulin; Kyrgyz mare; mare's mil More less